Abstract: Chitosan- cothxy pectin coating and cold storage ( 4℃) were combined to keep fresh and physiological metabolism of grapes. Grapes were coated with / without complex chitosan coating and then stored at room temperature or 4℃ up to 30 d for physicochemical quality evaluation. The results showed that complex chitosancothxy pectin coating at room temperature and cold storage without coating got the same effects on the physiological characteristics of kyoho grapes. Complex coating combined with cold storage could inhibite the respiration intensity to 7.1 ( CO2mg·kg- 1·h- 1) , delay repening time, decrease fruit decay rate, maintain higher VC content, increase the brightness and stabilize SSC content. Besides, the glass transition temperature of grapes coated with chitosan coating increased 9.75℃ which could benefit grape frozen preservation.