响应面法优化猕猴桃皮渣酶解取汁工艺
Optimization of enzymatic hydrolysis of Chinese gooseberry pomace by response surface methodology
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摘要: 以猕猴桃皮渣为原料,研究了果胶酶对皮渣出汁提高率的影响。在单因素分析的基础上,采用Box-Behnken中心组合实验,建立二次回归方程模型,模型相关系数R2=0.9636,对模型进行方差分析,确定了猕猴桃皮渣的最佳酶解工艺参数:酶解温度48.98℃、p H3.42、酶解时间3.32h、果胶酶用量0.26mg/g,在此条件下,皮渣的实际出汁提高率为(44.21±1.22)%,与理论值45.73%的相对误差为2.46%。Abstract: Chinese gooseberry pomace was used as raw materials in this paper to study the effect of pectinase on the increased ratios of juice yield by response surface methodology. Based on single factor experiments, the quadric regression equation model was established applying Box- Behnken center composite experimental design, and the correlation coefficient R2 of the model was 0.9636. Through variance analysis and solution of the model, the optimal condition for the hydrolysis of Chinese gooseberry pomace was determined as follows: enzyme temperature 48.98℃, p H3.42, enzyme addition 0.26 mg / g, and hydrolysis time 3.32 h. Under these conditions, the actual increased ratio of juice yield from Chinese gooseberry pomace was ( 44.21 ± 1.22) %, which was closed to the theoretical increased ratio of juice yield of 45.73% with a relative error of 2.46%.