产酸性蛋白酶菌株分子鉴定和酶学性质研究

Molecular identification and enzymology analysis of a acid protease producing strain

摘要: 本文采用透明水解圈法筛选到一株产酸性蛋白酶菌株PX8,以16S rDNA序列分析,确定其为Pectobacterium sp.菌株。通过改变酶促反应的pH、温度、金属离子和发酵时间等条件,采用正交实验分析了该菌株产酸性蛋白酶的特征。结果显示,在酶促反应液中Fe2+浓度为1.2mg/mL,反应温度为40℃,pH4,反应时间为10min时,酶活达到最大值34.625U/mL。

Abstract: In the paper, a bacterial strain PX8 with acid protease activity was screened by transparent hydrolysis ring method, and after 16 S rDNA sequence analysis, the strain was identified as Pectobacterium sp.By orthogonal test study concerning pH, temperature, metal ion of enzymatic reactions and fermentation time, acid protease enzyme activity of the strain was analyzed. The results showed, in the condition with 1.2mg /mL Fe2 +, reaction temperature 40℃, pH 4, reaction time 10 min, the enzyme activity reached its peak, 34.625 U /mL.