Abstract: The extraction of deer blood polypeptide was studied using trypsin hydrolysis with resh deer blood as aw material.This article took the degree of hydrolysis as index.The optimum conditions for enzymatic hydrolysis was determined on the basis of single factor and orthogonal experiment.The optimum conditions were the amount of enzyme 6%, pH9, the hydrolysis temperature 37℃, reaction time 4h, which the degree of hydrolysis reached46.72% ± 0.11% under this condition.And determination of antioxidant capacity of the peptides were performed.The high concentration of deer blood polypeptide had strong hydroxyl radicals scavenging ability and DPPH radical scavenging ability.