Abstract: This article firstly explored yields of chondroitin sulfate from pig lung trachea by comparing low concentration of sodium hydroxide extracting technology, enzymatic hydrolysis technology and low concentration of sodium hydroxide-enzymatic hydrolysis hybrid technology, then isolated and purified it by ethanol precipitation, DEAE- Sepharose Fast Flow anion exchange column chromatograph and Sephadex G- 75 gel column chromatograph.At last, structure and antioxidant of purified chondroitin sulfate was investigated by ultraviolet spectrum, infra- red spectrum, nuclear magnetic resonance and free radical scavenging activity. The results exhibited that enzymatic hydrolysis technology was the best and the yield of chondroitin sulfate was 22.10%.28 g chondroitin sulfate with 97% purify was obtained from 100 g crude product. The data of spectral analysis and antioxidant capability of chondroitin sulfate showed that its structure and antioxidant activity was similar to chondroitin sulfate from chicken keel cartilage and pig laryngeal cartilage.Thus, pig lung trachea can be used as good resource of chondroitin sulfate.