基于响应面法的蚕蛹蛋白降血脂肽的酶解条件优化

Enzymatic hydrolysis condition optimization of cholesterol-lowering silkworm pupa protein peptides based on response surface methodology

摘要: 基于响应面法对中性蛋白酶降解蚕蛹蛋白制备降血脂肽的酶解条件进行了优化。得到制备蚕蛹蛋白降血脂肽的最佳酶解条件为:酶量5.1%（w/w）,酶解时间5h,酶解温度52℃,酶解pH=7.0,底/水比（w/v）=3.9%。在此条件下,蚕蛹蛋白降血脂肽对3-羟基-3-甲基戊二酸单酰辅酶A还原酶（HMGR）的抑制率为45.24%。建立了基于蚕蛹蛋白降血脂肽酶解条件的优化模型。验证实验结果表明该模型的预测准确率达97.31%。

Abstract: Optimization of the hydrolysis conditions of cholesterol-lowering silkworm pupa protein peptides by response surface methodology was performed. The optimized conditions were as follows :enzyme dosage5.1%, hydrolyzing 5 hours at the enzymolysis temperature 52℃ and pH=7.0 with a ratio of substrate/water (w/v) 3.9%, the HMGR inhibitory rate of cholesterol-lowering silkworm pupa protein peptide reached 45.24%. A response surface optimized model based on the cholesterol-lowering silkworm pupa protein peptide extraction conditions was established. The verified test results showed the model with a 97.31% accurate rate.