五种小麦麸皮烷基酚类化合物体外抗肿瘤作用及初步的机制研究
Experimental study on antitumor effects of five Alkylphenol from wheat bran and its preliminary mechanism
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摘要: 目的:观察小麦麸皮中5种烷基酚类成分对体外肿瘤细胞的抑制作用,并初步探讨其作用机制。方法:MTT法测定5种化合物对人结肠癌细胞株LoVo、人肝癌细胞株HepG2和人乳腺癌细胞株MDA-MB-231增殖的影响及半数抑制浓度IC50。流式细胞仪检测5-十七烷基间苯二酚（5-heptadecylresorcinol）不同浓度对人结肠癌细胞株LoVo细胞周期的影响。结果:5-十七烷基间苯二酚对3种肿瘤细胞的增殖均有较强的抑制作用,5-十九烷基间苯二酚（5-nonadecylresorcinol）活性次之,其他化合物对肿瘤细胞的抑制作用较弱。5-十七烷基间苯二酚可将肿瘤细胞阻断于G2/M期,进而抑制肿瘤细胞增殖。结论:小麦麸皮中5种烷基酚具有一定的抗肿瘤活性,其活性可能与侧链长短相关。5-十七烷基间苯二酚具有较好的体外抑瘤作用,是一种有潜力的抗癌先导化合物,值得进一步深入研究。Abstract: Objective: The aim of this study was to observe the inhibitory effect of five alkylphenols from wheat bran in vitro, and explore its mechanism.Method: The proliferation and IC50 of five compounds on LoVo, HepG2, MDA-MB-231 cancer cell lines were assayed by MTT method in vitro.Flow cytometry was applied to analyze the effect of cell cycle with different concentrations of 5- heptadecylresorcinol in LoVo cancer cell lines. Result: The inhibition effects of 5- heptadecylresorcinol on all three tumor cell lines were significant, and 5- nonadecylresorcinol took second place.The other compounds had less toxicity to all three tumor cell lines than 5- nonadecylresorcinol.5-Heptadecylresorcinol could inhibit the proliferation of LoVo cells during G2/M period.Conclusion: The antitumor activity of five alkylphenols may be related to its length of side chains. Our results showed that5-heptadecylresorcinol possess good effects of inhibiting tumor cell proliferation in vitro.It may be a potential anticancer leading compound and need study further.
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