Abstract: The ZTC natural clarifying agent was employed to clarify brown sugar juice and the effect of some factors such as the adding sequence of ZTC clarifiers A and B, the adding proportion, the dosage, and the reaction temperature were investigated. To optimize the reaction condition, the experiment was arranged by orthogonal design in the base of analyzing of single factor. The optimum condition of reaction was obtained as follows:the adding sequence of ZTC natural clarifying agent was clarifier B before clarifier A. The adding proportion of B and A was 3∶1. The total dosage of A and B was 6mL. The reaction temperature was 40℃.Under these conditions, the clarification rate and the removal rate of turbidity may achieve 63.5% and 94.7%, respectively. The mechanism of flocculation was discussed via the theory of Zeta potential.