Abstract: Seven lactic acid bacteria strains isolated from high-quality pickle samples were screened to inhibit four kinds of spoilage microorganisms, which caused the pickle film. Lactobacillus plantarum SCP53, which had strong antimicrobial activity to tested spoilage microorganisms, was selected by oxford cup method and liquid culture (film, precipitation, liquid turbidity, and inhibition rate to spoilage microorganisms) . SCP53 and spoilage microorganisms were inoculated to make radish pickle, and the results indicated that SCP53 could prevent the formation of film and improved the quality of pickle.