Abstract: Based on the single factor experiment, using Box-Behnken experimental design method, the enzyme activity of laccase as response value, the fermentation conditions were optimized and the feasibility of experiment model was validated. The results indicated optimal ingredients including:the speed at 131r/min, temperature at27.6℃ and shake loading at 32mL/100mL. Under the optimal conditions, the strain of Pleurotus eryngii had higher degradation rate of phenanthrene. The degradation rate of phenanthrene reached 82.86% at the 12th day.