Abstract: With Momordica charantia as raw material, the process conditions of clarifying Momordica Charantia juice by chitosan were studied, and the formulation of the Momordica Charantia beverage was optimized.Using transmittance as index, the effects of chitosan amount, clarification time, pH and clarification temperature were investigated.Based on single factor experiments, orthogonal experiments were performed to optimize the process conditions.The optimal conditions were: chitosan amount 0.3g /L, clarification time 40min, pH5.5 and clarification temperature 30℃.Then single factor experiments and response surface analysis were combined with sensory evaluation to optimize the formulation of Momordica Charantia beverage.The best formulation was: 25.65%Momordica Charantia juice, 0.01% stevia sugar, 1% isomaltulose, 0.0094% citric acid.