Abstract: In this study, in order to optimize the enzymolysis conditions of pricklyash seeds antioxidative peptide, the degree of hydrolysis ( DH) and DPPH radical scavenging capacity of pricklyash seed protein antioxidative peptide as an indicator, the enzymolysis conditions, including enzymes type, enzyme concentration, substrate concentration, reaction temperature, time and so on were investigated.The results indicated that better effective was observed with Alcalase proteases hydrolysis pricklyash seed protein to prepare antioxidative peptides.Through the response surface methodology, enzymatic antioxidative peptide conditions of pricklyask seeds protein were optimized.The optimum conditions were substrate concentration of 5%, the temperature 55℃, pH 8.5, 4% of the amount of enzyme.The optimum method could be applied in the producing process of pricklyash seeds antioxidative peptide.Based on this condition, hydrolysate of pricklyash seeds protein performed higher antioxidative activity with 61.00% DPPH scavenging capacity, 12.00% degree of hydrolysis.