Abstract: The enzymolysis technology of Pinctada martensii meat was optimized by orthogonal array design methods, and effect of the enzymatic hydrolysate on serum biochemical indicators in animal SD rat was investigated. The optimum enzymolysis conditions were found to be enzymolysis at 70℃ and pH8.0 for 35min with 0.6% dosage of enzyme, under the condition, degree of hydrolysis of Pinctada martensii meat protein reached up to 54.79%. After deployed reagent of Pinctada martensii meat enzymatic hydrolysate was injected into the animal SD rat. PA, ApoA1 in blood serum significantly rose (p<0.05) , ALT, AST, ApoB, CK-MB, CRP, GLU, I-BiI in blood serum significantly dropped (p<0.05) , therefore, combining with previous research results, it was inferred that Pinctada martensii meat enzymatic hydrolysate possessed medical functions of nourishing liver and cardiovascular.