Abstract: Objective:An acute toxicity test was used to obtain a preliminary understanding of the safety of boiled Antarctic Krill and shrimp powder. Methods:Lab mice were classified into three categories. Two tested ones were fed with samples made by boiled Antarctic Krill and shrimp powderrespectively and the controlled ones were fed by SPF mice food. Seven consecutive days of observation and relevant record were needed.Results:Moisture content of samples, made by boiled Antarctic Krill and shrimp powder, were 14.14% and 15.47%, respectively, ash content were 12.54% and 10.93%, respectively. Furthermore, crude fat content of dry samples were 15.59% and 2.56%, respectively. And fluoride level were 1399.84mg/kg and 186.77mg/kg, respectively.After seven consecutive days of observation, there were no death cases. In fact, all the mice were normal. To conclude, there was nosignificant difference between the tested and controlled mice. The original maximum given dose of SPF mice food, boiled Antarctic Krill and shrimp powder were 290.89, 599.04g/kg and 62.71g/kg, respectively. The converted adult doses were 32.73, 64.22g/kg and 7.18g/kg, respectively. There was highly significant difference (p <0.01) between liver coefficient of tested and controlled mice, and There was no significant difference between others. Conclusion:The maximum tolerance dose of boiled Antarctic Krill and shrimp powder were above 599.04g/kg and 62.71g/kg, respectively. The safety of boiled Antarctic Krill needs further subchronicresearch as it may be harmful to liver.