Abstract: To research the application on food preservation of extract of Ampelopsis grossedntata, the leaves of A.grossedentata that were collected in March, July and November were taken as experimental materials, respectively. Escheridhia coli, Staphylococcus aureus, Bacillus thuringiensis, Sarcina lutea and Bacillus subtilis were taken as experimental strains. After the different bacteria were dealed with leaves extracts of A.grmsedntara, the inhibitory zone diameters were evaluated by using a disk- diffusion and the minimum bactericidal concentrations (MBC) were detected by double dilution method during the process of bacterial growth. Microorganisms in the food solution, which were related to different concentrations of the extracts, were quantified by colony counting method. The results showed the extracts from the leaves of A.grossedentata were collected in July had the strongestantibacterial effect on S. aureus, the inhibitory zone diameter reached27.0mm and MBC was only 5%. Bacteriostatic time for broth food could reach 140h when the concentration of the extracts was 4MBC. This indicated the extracts from the leaves of A.grossedentata had broad antimicrobial spectrum and good preservative effect on pork, milk, watermelon and soy milk.