米黑毛霉产凝乳酶固体发酵培养基优化

Optimization of culture media of Mucor mihei producing milk-clotting enzyme through solid-state-fermentation

摘要: 对米黑毛霉产凝乳酶固体发酵培养基进行优化,以提高凝乳酶的产量,为米黑毛霉凝乳酶的工业化生产提供技术依据。首先通过单因素实验,得到最佳碳源为葡萄糖,最佳氮源为硝酸铵,最佳产酶诱导物为乳清粉。在此基础上通过正交实验进一步优化,得到培养基外加成分的最优组合为葡萄糖浓度2.5%,硝酸铵浓度1.0%,乳清粉浓度2.0%。培养基优化后米黑毛霉的凝乳活力达到(3649.52±3.62)SU/mL,比优化前提高了54.5%。

Abstract: The culture medium was optimized to improve the yield of milk-clotting enzyme from Mucor miehei.The results of the single factor experiment showed that the best carbon source, the optimal nitrogen source, and the best enzyme inducer were glucose, ammonium nitrate, and whey powder, respectively.On this basis, through the orthogonal experimental design, the optimal extraneous components we obtained were as follows:2.5% glucose, 1.0% ammonium nitrate, and 2.0% whey powder. In the optimized medium, milk-clotting activity of Mucor miehei reached ( 3649.52 ± 3.62) SU /mL and increased by 54.5%.