Abstract: Tea, planted in Jiangxi province which was representation of hilly red soil region, was selected as research objects. By adding exogenetic Se and Zn which were regulated by N and P in soil, variation of Se, Zn, Al and Mn content, total phenolic content, as well as antioxidant ability in two leaves and a bud and old leaves were investigated. A new plant method which could decrease the content of Al and Mn and improve the natural quality of tea was expected to be explored. The results showed that the content of Se and Zn increased, which the highest growth rate respectively were 255.65% and 100.45%, while the content of Al and Mn decreased which the highest reduction rate were 39.27% and 35.85% through soil regulation. Moreover, the total phenolic and antioxidant ability of the tea increased.