酸胁迫对干酪乳杆菌细胞膜生理特性的影响

吴重德; 何桂强; 张娟; 周荣清; 堵国成; 陈坚

doi:10.13386/j.issn1002-0306.2014.05.081

酸胁迫对干酪乳杆菌细胞膜生理特性的影响

1. 四川大学轻纺与食品学院皮革化学与工程教育部重点实验室
2. 江南大学生物工程学院工业生物技术教育部重点实验室

基金项目:

国家自然科学基金(31301546)；

详细信息

作者简介:
吴重德 (1982-) , 男, 博士, 讲师, 研究方向:微生物生理学与生物化学。;

中图分类号: TS252.1
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出版历程
- 收稿日期: 2013-09-03

Effect of acid stress on membrane characteristics of Lactobacillus casei

College of Light Industry, Textile & Food Engineering, Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University
School of Biotechnology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University

摘要

摘要: 以干酪乳杆菌为研究对象,考察了酸胁迫对细胞膜生理特性的影响。研究结果表明,随着pH的降低,细胞膜侧向扩散系数降低,微黏度增加。同时分析细胞内膜透性和胞内金属离子浓度表明酸胁迫引起细胞膜透性增加。分析膜脂肪酸成分结果表明,酸胁迫引起不饱和脂肪酸含量增加,饱和脂肪酸含量降低,同时细胞膜脂肪酸不饱和度和膜脂碳链长度也相应增加。研究结果将为进一步解析干酪乳杆菌酸胁迫抗性机制奠定理论基础。
- 干酪乳杆菌 /
- 酸胁迫 /
- 细胞膜流动性 /
- 膜脂肪酸
Abstract: In this study, effects of acid stress on membrane characteristics were investigated by using Lactobacillus casei as initial strain. The results showed that lateral diffusion coefficient decreased and the micro- viscosity increased with the decrease of pH.In addition, analysis of the inner membrane permeability and concentrations of intracellular metal ions showed that acid stress led to increased permeability of cell membrane.The distribution of membrane fatty acids was investigated, and the results showed acid stress induced increased unsaturated fatty acids and decreased saturated fatty acids. Moreover, acid stress resulted in increased unsaturation degree and chain length of membrane fatty acids. Results presented in this study provided theoretical basis for further elucidation of the acid tolerance mechanisms employed by lactic acid bacteria.
- Lactobacillus casei /
- acid stress /
- membrane fluidity /
- membrane fatty acid

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参考文献(19)

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