乙酰胆碱酯酶抑制率法测定氨基甲酸乙酯的研究

刘丽斌; 黄秋婷; 彭程; 黄惠华

doi:10.13386/j.issn1002-0306.2014.05.054

乙酰胆碱酯酶抑制率法测定氨基甲酸乙酯的研究

1. 华南理工大学轻工与食品学院
2. 广州市酒类检测中心

基金项目:

广州市科技计划项目(201300000079)；

详细信息

作者简介:
刘丽斌 (1989-) , 女, 硕士研究生, 主要研究方向:食品科学。;

中图分类号: TS207.3
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出版历程
- 收稿日期: 2013-07-14

Determination of ethyl carbamate on acetylcholinesterase inhibion rate

College of Light Industry and Food Sciences, South China University of Technology
Alcohol Testing Center of Guangzhou

摘要

摘要: 本研究以乙酰胆碱酯酶对底物碘化硫代乙酰胆碱进行水解,根据氨基甲酸乙酯对乙酰胆碱酯酶的抑制作用,测定氨基甲酸乙酯。实验发现,经溴水溶液增敏后,显著提高了氨基甲酸乙酯对乙酰胆碱酯酶的抑制率,且其抑制程度与浓度在一定范围内呈线性关系,由此建立了一种快速测定氨基甲酸乙酯的酶抑制法。该法测定氨基甲酸乙酯的线性范围为1~7.5mg/L,相关系数r=0.9972,最低检出限为0.04177mg/L,RSD在1.82%~9.69%之间。该法所用试剂成本低,方法快速简单、条件温和易于控制、所需设备少,易于操作。本文为进一步开发基于酶抑制原理的酒精饮料及发酵食品中氨基甲酸乙酯快速检测方法技术及产品提供了相应的理论和技术支持。
- 乙酰胆碱酯酶 /
- 酶抑制率法 /
- 氨基甲酸乙酯
Abstract: The determination method of ethyl carbamate based on acetylcholinesterase inhibion was researched in this paper. It was found that inhibition rate of acetylcholinesterase activity by ethyl carbamate was significantly improved after the sensibilization by bromine solution. There was a linear relationship between inhibition rate of acetylcholinesterase activity and concentration of bromine solution in a certain range.The established determination method of ethyl carbamate had good linearity in a range of 1⁷.5mg /L and linear correlation reached 0.9972 and detection limit reached 0.04177mg /L.RSD was between 1.82% to 9.69%.The method which is easy to operate and control because of rapid, simple, and required less equipment under mild conditions, is low cost. This paper provide certain theoretical and technical support for further development of rapid detection technologies and products of ethyl carbamate based on enzyme inhibition in products of alcoholic beverages and fermented foods
- acetylcholinesterase /
- enzyme inhibition /
- ethyl carbamate

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参考文献(13)

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