Abstract: In order to optimize the extraction of amygdalin from Amygdalus pedunculatus pall to improve its value, the effects of operation parameters were discussed in this research. The response surface methodology was used to optimize influence of these three factors to the extraction. It indicated that the optimum conditions were as follows:the ethanol concentration of 90%, solid-liquid ratio 1∶6g/mL, extraction time 55min, the extraction percentage was 86.91%.