Abstract: In order to evaluate the influence of double-coating using alginate on the effectiveness of probiotic microcapsules, this study prepared three different microcapsules:alginate microcapsules, protein microcapsules, alginate-coated microcapsules, and compared these three microcapsules in three aspects, including:degradation rate of coating material, release rate of microencapslated probiotics and survival rate of microencapsulated probiotics. The results showed that the outer alginate membrane could significantly reduce the degradation role of pepsin on whey protein matrix. Beside, the coated microcapsules could delay the release rate of Bifidobacterium bifidum F-35 in simulated intestinal juice, but they provided the best protection for microencapsulated cells in sequential simulated gastrointestinal juice, the survival rate was up to 51.0%。