Abstract: Fish gelatin films incorporated with tea polyphenols nanoliposomes were obtained and their physicochemical properties including microstructure, mechanical property, permeability, optical absorption property and antioxidative activity were investigated. It was showed that the incorporation of tea polyphenols nanoliposomes had resulted in the modifying of microstructure, decreasing of tensile strength and water vapor permeability, and increasing percentage elongation at break and oxygen permeability of the gelatin films. Moreover, it was observed that the protective effect against fish oil oxidation and DPPH radical scavenging activity of the gelatin films had been enhanced, dued to the addition of tea polyphenols nanoliposomes.