响应面法优化超声辅助提取红皮云杉多酚工艺

Optimization of ultrasonic-assisted extraction conditions of polyphenol from Picea koraiensis Nakai by response surface methodology

摘要: 采用响应面法优化超声波辅助提取红皮云杉多酚工艺。在单因素实验的基础上,选择料液比、超声波功率、提取时间为自变量,以多酚得率为响应值,采用Box-Benhnken法设计三因素三水平的响应分析实验。结果表明,回归模型拟合度高,最佳工艺条件为:料液比1∶33、超声波功率301W、提取时间2.4h,乙醇浓度40%,温度60℃。在此条件下,红皮云杉多酚的得率为18.92%。

Abstract: The ultrasonic- assisted extraction conditions of polyphenol from Picea koraiensis were optimized by response surface methodology.Based on single factor test, a 3- factor, 3- level Box- Behnken experimental was adopted, including dependent variables ( ratio of material to liquid, ultrasonic wave power and extraction time) and independent variable ( yield of polyphenols) .The results showed that the regression model fit well with experimental data.The optimum conditions were ratio of material to liquid 1∶33, ultrasonic wave power 301W, extraction time 2.4h, ethanol concentration 40%, extraction temperature 60℃. Under these conditions, the yield of polyphenol was 18.92%.