正交设计优化新疆药桑桑叶总生物碱超声提取工艺

Optimization ultrasonic extraction of total alkaloids from Xinjiang black mulberry leafs based on orthogonal design

摘要: 优化超声法提取新疆药桑桑叶总生物碱。通过单因素实验和正交实验,研究提取时间、提取温度、料液比、超声功率和提取溶剂乙醇浓度对新疆药桑桑叶总生物碱提取效果的影响。超声提取法的优化工艺条件为提取时间20min,提取温度60℃,料液比1∶20（g/mL）,超声功率800W,乙醇浓度60%。在此条件下,提取新疆药桑桑叶总生物碱含量为4.64mg·g-1。新疆药桑桑叶中总生物碱含量高于普通桑叶,超声提取新疆药桑桑叶总生物碱,具有简单、省时、高效的优点。

Abstract: The ultrasonic extraction of total alkaloids from Morus nigra leaf was optimized by orthogonal design.Effects of ultrasonic time and temperature, solid-liquid ratio, ultrasonic power, ethanol (extraction solvent) volume fraction on the yield of total alkaloids from Morus nigra leaf were studied by single factor and orthogonal tests.The optimum ultrasonic extraction conditions were as follows:ultrasonic time 20min, extraction temperature 60℃, solid-liquid ratio 1∶20, ultrasonic power 800W and ethanol volume fraction 60%.Under these conditions, the average yield of the total alkaloids was 4.64mg·g-1.The ultrasonic extraction of total alkaloids from Morus nigra leaf was simple, fast and effective.