Abstract: The volatile oil from the stem and leaf of wild Chrysanthemum indicum was extracted by steam distillation, the chemical components of the volatile oil were separated and structurally identified by gas chromatography-mass spectrometry, and the antimicrobial activities of chemical components in the oils against the growth of five plant pathogenic fungi were evaluated by using growth rate method.A total of 32 compounds were identified in the volatile oil from the stem and leaf of wild Chrysanthemum indicum, the volatile oil had resistant to five plant pathogenic fungi, and showed higher inhibitory activities especially against Sclerotinia sclerotiorum, its inhibition rate was 81.63% in density 500μg/mL of the volatile oil.