Abstract: The influencing factors of lactic acid bacterias (LAB) for cholesterol-lowering which isolated from RuGao longevity population gut were studied in this paper.The ability of lowering cholesterol of 86 LAB were tested, and results showed that 5 strains which were Weissella cibaria, Weissella cibaria, Lactobacillus casei, Lactobacillus plantarum and Enterococcus faecium, and their cholesterol- lowering rate were more than 30.0%.Bile salt concentration, initial pH, cell survival state and viable count affected on the ability of lowering cholesterol were also researched.It was found that 5 strains had different suitable bile salt concentration of cholesterol- lowering in vitro;neutral pH was benefit to cholesterol-lowering compared to acidic pH;cell survival of LAB had a great influence on it's ability of lowering cholesterol;the ability of lowering cholesterol of most tested strains had direct relationship with their growth.