Abstract: Using xanthocera seeds as raw material, the oil were extracted by the method of ultrasound treatment and aqueous enzymatic which was assisted by NaCl to finish demulsification.Based on the single factor analysis, the xanthocera seeds oil extraction process was used to optimize the conditions of central composite design (BBD) .Besides, the fatty acid compositions of xanthocera seeds oil were analyzed with GC-MS.The extraction rate of the xanthocera seeds oil reached 89.85% under the optimum extracting conditions:ultrasonic power 150W, the extraction time 120min, cellulase 0.05%, NaCl addition amount was 0.10%, ratio of material to liquid 1 ∶ 3.5, enzymolysis temperature 30℃.GC- MS analysis results showed that the xanthocera seeds oil mainly contained 8 kinds of fatty acid, the unsaturated fatty acid content made up more than 97%, and the oil had good antioxidant activity.Ultrasound treatment and NaCl played a demulsifying role in extraction process improved the extraction yield of xanthocera seeds oil.