Abstract: The scavenging capability of several substances such as ascorbic acid, catechol, glucose and Bovine serum albumin (BSA) on nitrite was studied by aniline- sulfonic and hydrochloride acid- napHthylamine ethylenediamine measuring through spectropHotometry.Based on the single factor experiment that explored the common effect on the scanvenging rate of nitrite, a uniform design test were designed to identify the best conditions for these substances to nitrite scanvenging.The results showed that the nitrite scanvenging effect reached upmost while ascorbic concentration 0.7%, reaction time 15min, pH4 and temperature 4℃, respectively;Meantime, for catechol, its best scanvenging rate occured while same circumstance was 1%, 40min, pH2, 20℃ respectively.Besides, our experiment data testified that glucose and BSA perfomed less than 10% nitrite scanvenging.They had not a significant scanvenging effect on nitrition.