高温淀粉酶法制备大麦饮料的工艺研究
Study on preparation technology of barley beverage with thermostable amylase
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摘要: 以烘焙后的大麦为原料,采用高温淀粉酶酶解制备大麦饮料,并且对酶解参数进行研究。首先以原料利用率和总固形物含量为工艺指标,确定最佳料水比。然后在单因素实验的基础上,选取酶解温度、加酶量、酶解时间为自变量,以还原糖和总固形物含量为响应值,利用响应面优化方法(RSM)中的Box-Behnken方法研究自变量及其交互作用对响应值的影响。得到的最优条件为料水比1∶8,酶解温度90℃,酶解时间70min,加酶量60U/g。在此条件下,还原糖含量为27.2445mg/mL,总固形物含量为10.334g/100mL。Abstract: Barley beverage was prepared by thermostable amylase hydrolysis and the parameters were studied carefully, solid-liquid radio was optimized based on total solid content and utilization rate of raw materials.Three parameters including temperature, hydrolysis time and enzyme dosage were optimized using Box-Behnken central composite design and response surface methodology for achieving maximum of the total solid and reducing sugar content. The optimum hydrolysis condition was solid-liquid radio 1∶8, temperature 90℃, hydrolysis time 70min, total enzyme dosage 60U/g. Under these conditions, the reducing sugar content were27.2445mg/mL, the total solid content were 10.334g/100mL.