Abstract: Objective:There proteolytic enzymes, including pepsin, trypsin, papain and pepsin, were employed to hydroyze Seabuckthom seeds protein to produce the hydrolysates of antioxidant activity. Methods:The radical scavenging capacities and total antioxidant capacities in vitro antioxidant trials of three proteolytic peptides were compared and screened. Selected the best polypeptide from vitro antioxidant trials to do the oxidation experiment in vivo. Results:Among the three protein, the seabuckthorn seeds protein hydrolyzed produced by pepsin, had the highest antioxidangt activity. Result of animal oxidation experiment showed that antioxidant indicators of which was GSH and CAT were extremely significant (p<0.05) compared with the model group, that was seabuckthorn seed polypeptide hydrolyzed by pepsin. And lowering effect of the MDA indicators was significant (p<0.05) compared with the model group. Conclusion:There was a better antioxidant in hydrolysis seabuckthorn polypeptide by pepsin, compared with the other two polypeptides.