Abstract: Nutrition peptide powder was obtained by alkaline protease.The single factor experiment was designed with nitrogen yield and hydrolysis degree as index.On the basis of single factor, using the trials statistical software Design Expert8.0, with the degree of hydrolysis as response values, the optimal enzymatic hydrolysis conditions was as follows: enzyme solution temperature was 47℃, solid-liquid ratio was 1∶7g /mL, the pH was 9, add enzyme was 6200U /g.The degree of hydrolysis was reached to 17.47% under the optimal condition. Results showed that enzyme solution could significantly improve the degree of hydrolysis of proteins in horse bones meal.