PITC柱前衍生-液相色谱法测定西瓜中L-瓜氨酸含量

李志坚; 李高阳; 张群; 刘伟

doi:10.13386/j.issn1002-0306.2013.20.017

PITC柱前衍生-液相色谱法测定西瓜中L-瓜氨酸含量

李志坚^1,2,
李高阳^1,2,
张群¹,
刘伟¹

1. 湖南省农产品加工研究所
2. 湖南省食品测试分析中心

基金项目:

国家科技支撑计划(2012BAK17B17)；湖南省重大科技专项(2011FJ1002)；

详细信息

作者简介:
李志坚 (1977-) , 男, 硕士研究生, 研究方向:农产品质量安全。;

中图分类号: TS255.7;O657.72
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- 文章访问数: 129
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出版历程
- 收稿日期: 2013-03-26

Determination of L-citrulline in watermelon by high performance liquid chromatography with PITC precolumn derivation

Hunan Agricultural Product Processing Institute
Hunan Food Test And Analysis Center

摘要

摘要: 建立了RP-HPLC测定西瓜中L-瓜氨酸的方法。西瓜试样采用异硫氰酸苯酯（PITC）衍生后用液相色谱仪在245nm下进行测定,结果表明,L-瓜氨酸质量浓度在4.26²13.2μg/mL范围时,其质量浓度与峰面积呈良好的线性关系（R=0.9998）。L-瓜氨酸的最低检出限为1.1μg/mL,相关系数为0.9998,平均回收率为98.1%。参试西瓜试样中L-瓜氨酸含量在1.13².71mg/g之间,该方法前处理简单,灵敏、快速、可靠,适合用于西瓜样品中L-瓜氨酸含量的定量测定。
- L-瓜氨酸 /
- PITC /
- 液相色谱
Abstract: A method of RP-HPLC with phenylisothiocyanate (PITC) precolumn derivatization was established for the determination of L-citrulline in watermelon. The calibration curves of L-citrulline were linear over the range of 4.26²13.2μg/mL (R=0.9998) and the recovery rate was 98.1%. The content ranges of L-citrulline in watermelon were 1.13².71mg/g. The detection limit of the method was 1.1μg/mL. The method descried in this paper was fast, sensitive and reliable and it was most suitable for quantitative determination of L-citrulline in watermelon.
- L-citrulline /
- PITC /
- HPLC

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参考文献(11)

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