Abstract: The sterilization process of canned sea cucumber was studied.In the sterilization process, the steam temperature, canned soup temperature, cold point temperature of sea cucumber were measured accurately and the optimum canned sterilization condition was explored at the sterilization temperature of 121℃ with the evaluation indicators of the F value, hydroxyproline content, mass loss rate, TPA, and sensory score.The results showed that the F value of canned sea cucumber was calculated and the heating temperature curve of the sea cucumber, canned soup and sea cucumber cold point death rate curve was drawn;the optimum sterilization process condition was determined for 10min-12min-10min /121℃.Under this condition, the canned sea cucumber sterilization requirements could be satisfied, while avoiding the nutrient loss.