Abstract: Wheat gluten is a kind of nutritious, high quality and inexpensive plant-based proteins.There are four kinds of gluten means albumin, globulin, glutenin and gliadin in wheat.The separation of wheat gluten can use flour as raw material or the whole wet wheat directly.In this paper, the application of gluten in foodstuffs, chemical industry and feedstuff were summarized and the future research trend was also prospected.