多壁碳纳米管基质固相分散萃取-HPLC法测定蔬菜中的啶虫脒残留

杨敏; 方树桔; 谭伟; 李彬; 王红斌

doi:10.13386/j.issn1002-0306.2013.19.033

多壁碳纳米管基质固相分散萃取-HPLC法测定蔬菜中的啶虫脒残留

云南民族大学民族药资源化学国家民委-教育部重点实验室化学与生物技术学院

基金项目:

云南民族大学绿色化学与功能材料研究省创新团队项目(2011HC008)；云南省高校绿色化学新能源与材料科技创新团队项目(2011UY09)；

详细信息

作者简介:
杨敏 (1966-) , 女, 教授, 主要从事分离分析教学和研究工作。;

中图分类号: TS255.7;O657.72
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出版历程
- 收稿日期: 2013-01-23

Multi-walled carbon nanotubes and matrix solid phase dispersion extraction-HPLC for the determination of acetamiprid residue in vegetables

Key Laboratory of Ethnic Medicine Resource Chemistry, State Ethnic Affairs Commission & Ministry of Education, School of Chemistry and Biotechnology, Yunnan University of Nationalities

摘要

摘要: 建立了以多壁碳纳米管（MWCNTs）为萃取剂的基质固相萃取（MSPD）、HPLC测定蔬菜中啶虫脒残留的方法。将样品与工业级碳纳米管、无水硫酸镁混合研磨后装柱,并用乙酸乙酯洗脱,高效液相色谱法测定。采用C8柱（3.0mm×100mm,1.8μm）分离,甲醇-水（3∶2,v/v）为流动相,流速0.10mL/min,紫外检测波长250nm,进样量2.5μL,柱温为30℃。结果表明,该方法对蔬菜样品中啶虫脒的回收率为84.0%¹06.0%,精密度为0.1%¹2.5%,方法最低检出限为0.0085mg/L。该法简便、快速、准确。
- 基质固相分散萃取(MSPD) /
- 多壁碳纳米管(MWCNTs) /
- 高效液相色谱(HPLC) /
- 农药残留
Abstract: With multi-walled carbon nanotubes (MWCNTs) as extractant, a method based on MSPD, HPLC had been developed for the determination of acetamiprid residue in vegetables.Samples were homogenized with industrial-grade carbon nanotubes and anhydrous magnesium sulfate in a mortar, packed into a cartridge, eluted by ethylacetate, and analyzed by HPLC.The acetamiprid residue was separated on a C 8 (3.0mm ×100mm, 1.8μm) column, with methanol-water (3∶2, v /v) as the mobile phase at the flow rate of 0.10 mL /min, then detected by UV detector at wavelength 210nm.The injection volume was 2.5μL, and the column temperature was 30℃.The results showed that vegetable samples recoveries were between 84.0% ~ 106.0%, with the relative standard deviations (RSDs) of 0.1%¹2.5%.And the detection lmit was 0.0085 mg /L.The method was simple, rapid, and accurate.
- matrix solid phase dispersion extraction (MSPD) /
- MWCNTs /
- HPLC /
- pesticide residues

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