多壁碳纳米管基质固相分散萃取-HPLC法测定蔬菜中的啶虫脒残留

杨敏; 方树桔; 谭伟; 李彬; 王红斌

doi:10.13386/j.issn1002-0306.2013.19.033

多壁碳纳米管基质固相分散萃取-HPLC法测定蔬菜中的啶虫脒残留

云南民族大学民族药资源化学国家民委-教育部重点实验室化学与生物技术学院

基金项目:

云南民族大学绿色化学与功能材料研究省创新团队项目(2011HC008)；云南省高校绿色化学新能源与材料科技创新团队项目(2011UY09)；

详细信息

作者简介:
杨敏 (1966-) , 女, 教授, 主要从事分离分析教学和研究工作。;

中图分类号: TS255.7;O657.72
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出版历程
- 收稿日期: 2013-01-23

Multi-walled carbon nanotubes and matrix solid phase dispersion extraction-HPLC for the determination of acetamiprid residue in vegetables

Key Laboratory of Ethnic Medicine Resource Chemistry, State Ethnic Affairs Commission & Ministry of Education, School of Chemistry and Biotechnology, Yunnan University of Nationalities

摘要

摘要: 建立了以多壁碳纳米管（MWCNTs）为萃取剂的基质固相萃取（MSPD）、HPLC测定蔬菜中啶虫脒残留的方法。将样品与工业级碳纳米管、无水硫酸镁混合研磨后装柱,并用乙酸乙酯洗脱,高效液相色谱法测定。采用C8柱（3.0mm×100mm,1.8μm）分离,甲醇-水（3∶2,v/v）为流动相,流速0.10mL/min,紫外检测波长250nm,进样量2.5μL,柱温为30℃。结果表明,该方法对蔬菜样品中啶虫脒的回收率为84.0%¹06.0%,精密度为0.1%¹2.5%,方法最低检出限为0.0085mg/L。该法简便、快速、准确。
- 基质固相分散萃取(MSPD) /
- 多壁碳纳米管(MWCNTs) /
- 高效液相色谱(HPLC) /
- 农药残留
Abstract: With multi-walled carbon nanotubes (MWCNTs) as extractant, a method based on MSPD, HPLC had been developed for the determination of acetamiprid residue in vegetables.Samples were homogenized with industrial-grade carbon nanotubes and anhydrous magnesium sulfate in a mortar, packed into a cartridge, eluted by ethylacetate, and analyzed by HPLC.The acetamiprid residue was separated on a C 8 (3.0mm ×100mm, 1.8μm) column, with methanol-water (3∶2, v /v) as the mobile phase at the flow rate of 0.10 mL /min, then detected by UV detector at wavelength 210nm.The injection volume was 2.5μL, and the column temperature was 30℃.The results showed that vegetable samples recoveries were between 84.0% ~ 106.0%, with the relative standard deviations (RSDs) of 0.1%¹2.5%.And the detection lmit was 0.0085 mg /L.The method was simple, rapid, and accurate.
- matrix solid phase dispersion extraction (MSPD) /
- MWCNTs /
- HPLC /
- pesticide residues

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参考文献(14)

[1]	唐振华.新烟碱类杀虫剂的结构与活性及其药效基团[J].现代农药, 2002 (1) :16.
[2]	苏婷, 侯如燕, 赵秀霞, 等.茶叶、茶鲜叶及茶汤中啶虫脒残留的检测[J].食品与发酵工业, 2011, 37 (10) :174-177.
[3]	李娟, 孙程鹏, 侯颖, 等.超高效液相色谱-电喷雾串联质谱测定蔬菜水果中27种常见农药残留[J].现代农药, 2011, 10 (3) :31-35, 38.
[4]	李广领, 姜金庆, 王自良, 等.气相色谱法检测卷心菜中啶虫脒和吡虫啉残留[J].西北农业学报, 2010, 19 (9) :71-74.
[5]	康燕玉.UPLC法快速测定白菜中啶虫脒残留量[J].福建分析测试, 2010, 19 (4) :55-58.
[6]	Qi Zhang, Defu Cai, Jianhua Liu.Matrix solid-phase dispersion extraction coupled with HPLC-diode arraydetection method for the analysis of sesquiterpene lactones in root of Saussurea lappa C.B.Clarke[J].Journal of Chromatography B, 2011, 879:2809-2814.
[7]	张立金, 王晓.基质固相分散—气相色谱—质谱法检测蔬菜中的农药多残留[J].山东科学, 2010, 23 (1) :11-14.
[8]	Chambers A, park C, Baker R K, et al.Hydrogen Storage in GraPnite Nanofibers[J].Journal of Physical Chemistry B, 1998, 102:4253-256.
[9]	张茜, 张公信, 李晓芬, 等.碳纳米管丝网印刷电极测定鞣花酸[J].云南民族大学学报:自然科学版, 2012, 21 (3) :167-169.
[10]	赵海香, 史文礼, 孙大江, 等.多壁碳纳米管-固相萃取分析有机磷农药残留[J].分析化学 (FENXIHUAXUE) 研究报告, 2009, 37 (10) :1479-1483.
[11]	赵海香, 贾艳霞, 丁明玉, 等.多壁碳纳米管固相萃取净化气相色谱法分析蔬菜中有机氯和拟除虫菊酯农药残留[J].色谱, 2011, 29 (5) :443-449.
[12]	李权龙, 袁东星.多壁碳纳米管用于富集水样中有机磷农药残留的研究[J].厦门大学学报:自然科学版, 2004, 43 (4) :531-535.
[13]	王学翠, 刘冰, 张璐鑫, 等.多壁碳纳米管基质固相分散-高效液相色谱测定牛奶中6种四环素[J].食品科学, 2011, 32 (14) :206-209.
[14]	GB 2763-2012食品安全国家标准食品中农药最大残留限量[S].