Abstract: The paper measured the influence of water, maltodextrin, arabic gum, β-cyclodextrin on the royal jelly eutectic point by resistance method.The results showed that the addition of maltodextrin improved royal jelly eutectic point and increased with increasing concentration of maltodextrin.gum arabic reduced the eutectic point, but it increased with increasing concentration.When β-cyclodextrin concentration in 0.5%, the eutectic point was improved, but with the increase of β-cyclodextrin concentration, royal jelly eutectic point was reduced.The research provided certain basis for freeze-dried study on shorten the freeze time and reduced energy consumption.