Abstract: The difference of liver meat quality, routine nutrition quality, the content and composition of amino acid from different crossbred cattle was studied.Cattle livers were chosen from 3 groups of crossbred cattle which were Angus cattle and Qinchuan cattle, Angus cattle and Hexi cattle, Luxi cattle and Limousin cattle, the national standard and conventional methods were used to determinate the liver meat quality, routine nutrition quality and the content of amino acid.Result showed, the liver from 3 kinds of crossbred cattle had a dark red color, the pH values were 6.17, 6.44, 6.22, the cooked meat rates were 82.46%, 77.03% and 80.38%, and have a good processing performance, protein content were 20.11, 18.82 and 19.01g/100g, amino acid contents were 19.68, 17.68, 17.63g/100g, and essential amino acid contents were 7.30, 6.46, 6.44g/100g, also had an abundant of amino acids of EAA, the EAAS were all above or close to the model proposed of FAO.Although there were some differences that existed in liver meat quality and nutrition quality between different crossbred cattle, the bovine livers could be the high quality by-products and had a high value to develop ment.