响应面法优化黑灵芝膳食纤维提取工艺

Extraction of dietary fiber from Ganoderma atrum with response surface optimization

摘要: 以黑灵芝为原料,采用酶法和化学法联用,从黑灵芝中提取出可溶性膳食纤维和不可溶性膳食纤维,借助响应面设计分析,考察酶解温度、酶解时间、料液比和碱提pH、碱提温度、碱提时间分别对黑灵芝可溶性膳食纤维(SDF)、不可溶性膳食纤维(IDF)得率的影响。结果表明,最佳提取工艺条件分别为:酶解温度98℃、酶解时间103min、料液比1∶32和碱提pH10、碱提温度48℃、碱提时间41min,在此条件下黑灵芝SDF得率为1.08%,IDF得率为88.68%。

Abstract: Response surface methodology was applied to optimize the extraction conditions ofsoluble dietary fiber (SDF) and insoluble dietary fiber (IDF) from Ganoderma atrum.Effects of enzymolysis temperature, reaction time and material-liquid ratio on the yield of SDF and alkaline extraction pH, temperature and time on the yield of IDF were investigated.Results exhibited that the optimum extraction conditions were as follows:enzymolysis temperature and reaction time was 98℃ and 103min, solid-liquid ratio was 1∶32, alkaline extraction pH, temperature and time was 10, 48℃, 41min.The yield of SDF and IDF was 1.08% and 88.68%, respectively, under optimized conditions.