Abstract: Research about anti- aging effect of squalene and vitamin E to skin where they were mixed with 10% fresh pigskin slurry with a volume of 20, 40, 60, 80, 100μL of squalene.The activity of superoxide dismutase (SOD) , glutathione peroxidase (GSH-Px) and catalase (CAT) of the pig skin slurry were evaluated, the absorption value reacted indirectly the change of protein carbonyl groups and protein hydroperoxides content, and the blank as a control.The results showed that squalene could remarkably improve SOD, GSH-Px and CAT activity of the pig skin.It also significantly reduced protein carbonyl groups and protein hydroperoxides content of the pig skin, and the effect of squalene was better than vitamin E.Taken togrther results indicated that squalene had anti-aging effect on the skin.