Abstract: An investigation studied on the amantadine residue in chicken product was carried out by HPLC-MS/MS. In this assay, 19 of 103 samples were detected to contain amantadine residue. The concentration of amantadine in the chicken livers, wing, drumstick and was approximately 5.5, 32.6～92.4, 11.4～25.3μg/kg, respectively. No residue was detected in chicken breast. Based on the result, we were able to reach the conclusion that there was partially exist of the illegal use of amantadine in the chicken husbandry and different part of chicken body has not the same possibility of confer the amantadine residue. The further supervise in the chick breeding is needed.