Abstract: Objective:To explore the antioxidant effect of silkworm pupa peptides (SPP) in aging mice induced by D-gal. Methods:Kunming mice were randomly divided into nine groups, control group, model group, and SPP low-, medium- and high-dose groups, silkworm pupa protein group, V C group, glutathione group, fish collagen peptides group. Except the control group, all the other groups were given intraperitoneal injection of D -gal, accompanying gavage the corresponding materials. Activities of CAT, SOD, GSH -Px, T -AOC, the content of MDA in brain and liver, the index of thymus and spleen and serum white blood cell count were determined after a trial period of 42 days. Results:Activities of CAT, SOD, GSH-Px and T-AOC exhibited a significant increase in liver and brain (p<0.05 or p<0.01) for SPP-M and SPP-H. Furthermore , the content of MDA showed a remarkable reducing trend (p<0.05 or p<0.01) . Moreover, SPP groups could ease the immunocompromised symptoms in D-gal-induced aging mice. In comparison with fish gelatin peptides and silkworm pupae protein, the SPP had a more pronounced effect on improving oxidative damage in mice, which was similar to GSH. Conclusion:SPP has a positive antioxidant activities and immunologic function in vivo as expected. This paper also provided the basic data for the utilization of these silkworm pupa resources.