苋籽ACE抑制肽的大孔树脂分离纯化

Macroporous resin purification of Amaranth angiotensin-I converting enzyme (ACE) inhibitory peptides

摘要: 比较6种大孔树脂对苋籽ACE抑制肽的吸附-解吸效果,从中筛选出合适该活性肽分离纯化的树脂,并对其吸附-解吸工艺进行优化。结果表明,DA201-C树脂最适合苋籽ACE抑制肽的纯化,在样品质量浓度10mg/mL,pH为5,上样量1BV,流速6mL/min时,树脂的吸附效果最佳,吸附率达83.69%,再用5BV体积分数75%乙醇,以5mL/min的流速进行洗脱,此时几乎把吸附的多肽全部洗脱下来,解吸率为98.69%。经树脂纯化,样品的蛋白纯度为89.47%,脱盐率为88.86%,短肽含量提高了20.96%,ACE抑制活性提高了27.91%。

Abstract: The adsorption and desorption rates of 6 types of macroporous resins towards Amaranth angiotensin-I converting enzyme inhibitory peptides were investigated. Among the study, DA201-C resin was the most suitable one to purify the bioactive peptides. When 1BV of 10mg/mL and pH5 sample was flowed at a speed of 6mL/min through DA2-1C resin, the adsorption effect was best with a adsorption rate reaching 83. 69%. Then the adsorbed peptides could be almost exactly eluted with 5BV of 75% (V/V) ethanol at 5mL/min. After the purification, the protein content and salt rejection rate were 89. 47% and 88. 86% respectively, as well as the oligopeptides content and ACE inhibition were changed by 20. 96% and 27. 91%.