大孔吸附树脂纯化百合磷茎中多酚类化合物

Study on technology of separation and purification for polyphenols from lilium brownii by croporous absorption Resin

摘要: 考察了8种大孔吸附树脂对百合磷茎中多酚类化合物的吸附分离性能,确定大孔吸附树脂纯化百合中多酚类化合物的工艺条件。结果表明NKA-2树脂具有较佳的吸附分离性能,其纯化多酚的工艺条件为:上样液中多酚类化合物的质量浓度为1.25mg/mL,pH3.0,流速4BV/h,吸附后再用5倍树脂体积的50%乙醇以4BV/h的流速解吸,解吸率83.46%。纯化后产品中多酚类化合物含量为6.68%。

Abstract: In order to set up the technology for adsorbing and separating polyphenols from Lilium Brownii with macroporous adsorption resin, 8 types of resin were investigated. The results showed that NKA-2 resin was the best resin for adsorbing and separating polyphenols in the following technological conditions:the concentration of sample of extract, 1. 25mg/mL;pH3. 0;the flow rate of fluid, 4BV/h;the eluting regent, 50% ethanol, 5 folds the volume of the resin;elution rate, 4BV/h, desorbing rate was 83. 46%, the content of polyphenols in product was 6. 68%.