HPLC法测定婴儿配方奶粉中维生素A含量的不确定度评定

HPLC method for the uncertainty of vitamin A determination in infant’s milk

摘要: 参照GB5413.9-2010测定婴儿奶粉中维生素A的含量。通过对测定过程中的不确定度来源进行分析,评定出各不确定度分量,最后计算出合成标准不确定度和扩展不确定度,结果表明试样中维生素A含量为15.013μg/g,扩展不确定度为1.496μg/g。不确定度分量结果显示,标准曲线拟合产生的不确定度在不确定度合成中贡献最大,因此在对婴儿配方奶粉中维生素A进行测定时,应对标准曲线的拟合过程加强控制,确保结果的准确性。

Abstract: To base on GB 5413.9-2010, the vitamin A content in infant’s milk was determined.By the analysis of the source of uncertainty during the entire determination process, the individual uncertainty was evaluated, and finally calculates synthetic standard uncertainty and expanded uncertainty.The results showed that the vitamin A content in the sample was 15.013μg/g, and the expanded uncertainty was 1.496μg/g.The individual uncertainty showed that the standard curve fitting made the greatest contribution to the combined standard uncertainty.So, the standard curve fitting process would be controled to ensure the accuracy of the results.