银条α-低聚半乳糖的免疫调节作用研究

Immunomodulatory effect of alpha-galactooligosaccharides from Stachys Floridana schuttl.ex Benth

摘要: 以银条为原料,通过乙醇浸提与D101大孔吸附树脂初步纯化,制备得α-低聚半乳糖（α-GOS）,并采用免疫抑制小鼠模型评价其免疫调节活性。结果表明:银条α-GOS含量为76.39%,主要由蔗糖、棉籽糖和水苏糖组成,其中水苏糖约占总α-GOS的86.03%。银条α-GOS能显著地提高环磷酰胺诱导的免疫功能低下小鼠脾脏指数、迟发型超敏反应的耳肿胀度和血清中溶菌酶的活性,且这种变化与银条α-GOS呈现剂量依赖关系。因此,银条α-GOS具有良好的免疫调节活性。

Abstract: An alpha-galactooligosaccharides (α-GOS) sample was prepared from Stachys floridana Schuttl.ex Benth by ethanol extraction and D101 macroporous adsorption resin purification, and immunomodulating activities in vivo of α-GOS were evaluated by using immunosuppressed mice model experiments.The results showed that α-GOS with a purity of 76.39% mainly composed of sucrose, raffinose and stachyose, and stachyose accounted for about 86.03% of α-GOS.The α-GOS could significantly increase the index of spleen, activity of Lysozyme in serum, the swelling rate of ear in delayed-type hypersensitivity of immune suppress mice induced by cyclophosphamide in a dose-dependent manner.Therefore, α-GOS from Stachys floridana Schuttl.ex Benth had a good immunoregulatory activity.