滁菊活性成分的低温浸提及其对α-葡萄糖苷酶活性的抑制作用

Study on low temperature extraction of Chuju Chrysanthemum morifolium and its α-glucosidase inhibitory activity

摘要: 为优化滁菊活性成分低温浸提工艺,以滁菊总多酚及总黄酮提取率为指标,考察时间、温度、液固比等因素对提取效果的影响,并采用正交实验优化浸提工艺。结果表明,滁菊低温浸提最佳工艺条件为:提取温度为60℃、液固比为30:1、浸提时间为50min,在此条件下,总多酚及总黄酮提取率分别为5.905、53.138mg/g。滁菊浸提液对α-葡萄糖苷酶活性具有抑制作用,IC₅₀为32μg/mL。

Abstract: To optimize the low temperature extraction process of Chuju Chrysanthemum morifolium, the effects of extracting time, extracting temperature and the ratio of liquid to solid on extraction rate were explored by one-factor-at-a-time method.The extracting process was optimized by orthogonal design.The extraction rates of total polyphenol and total flavonoids were 5.905, 53.138mg/g under the optimized condition that extracting temperature was 60℃, the ratio of liquid to solid was 30∶ 1, and extracting time was 50min.The extract showed potent inhibitory activities against α-glucosidase with IC₅₀ value of 32μg/mL.