耐镉肠杆菌的分子鉴定和抗性分析

Molecular identification and Cd～ (2+) resistance analysis of Enterobacter sp.

摘要: 分离到一株耐镉肠杆菌NP23,对其进行了分子鉴定和抗性研究,分析菌株在不同镉浓度条件下菌体的生长状况以及对Fe²⁺,Cu²⁺,Mn²⁺,Zn²⁺等重金属抗性,利用SDS法进行质粒消除分析。结果显示,菌株NP23是Enterobacter sp,高浓度的镉使该菌株生长延滞期变长,进入对数期后的生长量相对较低,采用SDS法进行质粒消除,初步确定NP23的镉抗性基因在基因组上,NP23对所分析的四种重金属都有一定抗性,其中对Zn²⁺和Mn²⁺的抗性较强,在3200mg/L以上。

Abstract: In the paper, a cadmium-resistance Enterobacter NP23 was separated, and its molecular identification and cadmium resistance were analyzed.Cell growth curve was studied in the liquid medium of different cadmium concentration, Fe～ (2+) , Cu～ (2+) , Mn～ (2+) and Zn～ (2+) resistance of NP23 was analyzed, and plasmid elimination with SDS method was studied.The results showed that the strain NP23 was an Enterobacter sp.The results revealed that lag phase was extended and biomass in logarithmic phase was relatively low at high cadmium concentration.Plasmid elimination with SDS method showed NP23 cadmium resistance gene is located in the genome.Analysis of Fe～ (2+) , Cu～ (2+) , Mn～ (2+) and Zn～ (2+) resistance of NP23 showed that the strain had a certain resistance of these four heavy metals, among which the strain had a high MIC of Zn～ (2+) and Mn～ (2+) , 3200mg/L or more.