Abstract: Bacteriocin-producing stain RX-6 was isolated from Spanish traditional Salami sausages. After eliminating some interference factors such as the organic acids, hydrogen peroxides and strain cells, the antimicrobial activity of the fermentation broth of strain RX-6 remained the same. By ammonium sulphate precipitation and dialysis, the activity was significantly enhanced. However, the substance was inactivated when treated with proteinase K. These results indicated the nature of antibacterial substance produced by strain RX-6 was protein. Based on morphological, physiological and biochemical characteristics, 16S rRNA gene sequence and phylogenic analysis, strain RX-6 was identified as Lactobacillus curvatus. Results obtained from characteristics analysis of the bacteriocin produced by strain RX-6 showed this bacteriocin still remained activated after the heat treatment at 121℃ for 20min and the pH activity range was 3 to 10, which indicated that it had strong heat stability and acid-base tolerance. Also, the bacteriocin could be inactivated by pepsin and trypsin, and partially inactivated by acid proteinase, which suggested that its use safety was very high. In addition, the bacteriocin had a broad inhibitory spectrum and could show inhibitory activity against Listeria spp., Staphylococcus spp., Pseudomonas spp. and Escherichia coli, which indicated that the bacteriocin had a potential application as natural food bio-preservatives in food industry.