Abstract: The optimum preparation technology and preliminary characterization of Eucommia ulmoides seed oil β-cyclodexitrin inclusion complex were explored in this paper. The optimization of the preparation technology for the inclusion complex was studied by means of orthogonal test with the weighted average of inclusion rate and product yield (comprehensive score) as the appraisement criteria. The structure of the inclusion complex was preliminary characterized respectively by differential scanning calorimetry (DSC) , ultraviolet spectrophotometry (UV) and microscopic imaging. The result showed that the optimum preparation technology were as follows:β-cyclodextrin∶Eucommia ulmoides seed oil (g/mL) =8∶1, inclusion temperature 60℃, inclusion time 2.0h. The analysis results of DSC, UV and microscopic imaging indicated that the inclusion complex was really a kind of new phase.