Abstract: Single factor and orthogonal experiment were used to study on the extraction technology of flavonoids from Elaeagnus angustifolia L., and the ability of scavenging DPPH.and rancimat method were used for antioxidant activity evaluation, the antibacterial activities was measured by Nitrocefin test.The result showed that the optimum extraction conditions were ratio to solvent 1:18 (g /mL) , ultrasonic time 10min, ethanol concentration 60%, ultrasonic power 320W.Under these extraction conditions, the yield of flavonids was to 5.28%.Flavonoids of Elaeagnus angustifolia L.had strong antioxidant activity, and the antioxidant activity was stronger than V C at the same concentration, and had strong anti-microbial activity.The MIC was 1.0g/L against E.coli.and Staphylococcus spp., and that was 1.5g/L against Bacillus spp., Penicillum, Mucor racemosus, Aspergillus niger.